BBa_K137020

BBa_K137020 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K137020
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Allen Lin
Date created: 2008-07-20 11:00:00
Date modified: 2015-05-08 01:10:08

GFP with (AC)21 repeat after start codon



Types
DnaRegion

Roles
Reporter

engineered_region

Sequences BBa_K137020_sequence (Version 1)

Description

GFP with (AC)21 repeat after start codon

Notes

With 21 AC repeats, the GFP sequence is in frame with respect to the start codon, so a functional GFP should be transcribed. The number of AC repeats was limited by maximum length of synthesized primers.

Source

The dinucleotide repeat was placed into synthesized primers that bound to the existing part I13401, and then a PCR reaction was run.

Sequence Annotation Location Component / Role(s)
ATG
AC repeat
1,1
4,43
feature/start start_codon
feature/misc sequence_feature
igem#experience
Issues
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K137020/1