BBa_K137047

BBa_K137047 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K137047
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Allen Lin
Date created: 2008-07-20 11:00:00
Date modified: 2015-05-08 01:10:09

250 bp inverted tetR promoter



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_K137047_sequence (Version 1)

Description

Inverted tetR promoter with noncoding, spacer DNA downstream of it to make the total length 250 bp. The promoter faces the 5' direction.

Notes

This part is in a series of inverted tetR promoters that have total lengths of 150, 250, 350, 450, 650, and 850 bp. We chose the region upstream of tetR in part Q04400 to be the noncoding DNA segment. This noncoding segment consists of the 3' end of tetR and B0015. None of the parts in this series was long enough to include the start codon of tetR, so a functional tetR protein should not be transcribed.

Source

Primers were synthesized to bind to part Q04400, and then a PCR reaction was run.

Sequence Annotation Location Component / Role(s)
tetR promoter
1,54
promoter feature/promoter
igem#experience
Works
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K137047/1