YenR

BBa_K1381004 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1381004
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Stephanie Herman, Gunta Celma, Megha Biradar, Christoffer Andersson and Martin Friberg
Date created: 2014-10-01 11:00:00
Date modified: 2015-05-08 01:10:13

B0034-YenR



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K1381004_sequence (Version 1)

Description

YenR is an activator originated from Yersinia enterocolitica. It induces the expression of the yenbox_WT, the yenbox fused with a wildtype promoter from Y. enterocolitica.
Both YenR and the yenbox_WT are parts in the Yen system, which is a homolougous system to the Lux system. It is the quorum sensing system derivated from Y. enterocolitica and it senses the presence of Y. enterocoliticas unique quorum sensing molecules, the OHHL:s (3-oxo-hexanoyl homoserine lactone). When the OHHL enters the host, it interacts with YenR, making it loose its shape and thereby preventing it from binding to the yenbox. So in the presence of Y. enterocolitica the induction of the expression will be lost.

Notes

The coding sequence of YenR was codon optimized for E. coli.

Source

The source of this part is Yersinia enterocolitica.

Sequence Annotation Location Component / Role(s)
B0034
YenR
1,12
19,753
feature/rbs ribosome_entry_site
feature/cds CDS
igem#experience
None
 
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1381004/1