AraC RBS

BBa_K1392935 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1392935
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Şeniz Y??ksel
Date created: 2014-09-28 11:00:00
Date modified: 2015-05-08 01:10:15

AraC-Pbad - Arabinose inducible regulatory promoter/repressor unit+ RBS



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K1392935_sequence (Version 1)

Description

This part contains the promoter and its coding sequence for the repressor AraC This is transcribed in upstream direction by binding to L(+)-arabinose, AraC changes its conformation.

Notes

We could easily use this part by binding it with a RBS, to produce an inducible regulatory promoter/repressor unit.

Source

In Escherichia coli, the AraC protein represses transcription from its own promoter, PC, and when it is associated with arabinose, it activates transcription from three other promoters, PBAD, PE, and PFGH. Expression from all four of these promoters is also regulated by cyclic AMP-catabolite activator protein; however, the arrangement of the protein binding sites is not identical for each promoter.

Access Instance Definition
public
public
BBa_K808000
BBa_B0034
Ara
BBa_B0034
igem#experience
Issues
 
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1392935/1