Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Şeniz Y??ksel Burak Kızıl Helin Tercan
Date created: 2014-10-06 11:00:00
Date modified: 2015-05-08 01:10:15
araC-Pbad + RBS + tetR- LVA
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1392938_sequence (Version 1) |
Description
This part is a composite structure which contains a promoter, RBS side and and a tetracycline repressor from transposon Tn10. It is transcribed in the opposite direction for the coding sequence of the repressor AraC. Through the binding to L+arabinose, the conformation of AraC changes.This causes the protein to diffuses from the DNA thereby inducing transcription. Through the RBS, the definition of the RBS efficiency could be observed.Notes
L(+) - Arabinose is in sugar form and harmlessThe designed promoter has low background activity, so the membrane proteins will not damage the cells before induction.
Source
References://parts.igem
Schleif, R. (2000). "Regulation of the L-arabinose operon of Escherichia coli." Trends Genet 16(12): 559-565.
Ren, H., D. Yu, et al. (2009). "High-level production, solubilization and purification of synthetic human GPCR chemokine receptors CCR5, CCR3, CXCR4 and CX3CR1
http://openwetware.org/wiki/Titratable_control_of_pBAD_and_lac_promoters_in_individual_E._coli_cells#pBAD_promotersOpenWetWare
http://www.ncbi.nlm.nih.gov/pubmed/7768852?dopt=Abstract