Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jiajun Tan
Date created: 2014-10-01 11:00:00
Date modified: 2015-07-22 02:48:08
OprF(Ala196)+CBP
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1393001_sequence (Version 1) |
Description
The OprF is a major outer membrane protein of Pseudomonas aeruginosa. This protein functions as a nonspecific porin to allow the passage of small hydrophilic molecules, plays a structural role in maintaining cell shape and outer membrane integrity, and is required for growth under low osmolality. The structure of OprF has been proposed to consist of three domains, the N-terminal forming h-barrel structure, a loop or hinge region, and the C-terminal associated with peptidoglycan. Based on the predicted secondary structure and information found in the literature, we chose Ala196 as potential fusion sites for displaying CBP. CBP is the abbreviation of copper binding peptide made up of seven amino acid.Notes
Based on the predicted secondary structure and information found in the literature, we chose Val(188) as potential fusion sites for displaying CBP. CBP is the abbreviation of copper binding peptide made up of seven amino acid.Source
The OprF gene is from Pseudomonas aeruginosa.| Sequence Annotation | Location | Component / Role(s) |
| oprF to Ala196 CBP | 1,588 589,609 | CDS feature/protein feature/binding non_covalent_binding_site |