Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Gaurav Sinsinbar,Nikhil Patidar ,Chandel Angad Singh
Date created: 2014-10-09 11:00:00
Date modified: 2015-05-08 01:10:15
sqr gene (sulfide quinone reductase) under constitutive promoter
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1395002_sequence (Version 1) |
Description
The sqr gene encodes a protein of 427 amino acid residues with a theoretical molecular weight of 47 kDa. The sqr gene is expressed under a constitutive promoter and this enzyme converts the sulphide (S-2) to elemental Sulfur. Its expression is based on the availability of RNA polymerase holoenzyme and the expression is not affected by any transcription factors and is 3A assembly (RFC10) compatible. The Constitutive Promoter (BBA_J23119) is the "consensus" promoter sequence and the strongest member of the constitutive promoter family developed by John Christopher Anderson of UC Berkeley. This promoter can be used to tunes the expression level of constitutively expressed parts.This part codes for the protein sulfide quinone reductase which is a FAD dependent oxidoreductase. Sulfide-quinone reductase (SQR),an ancient flavoprotein, is obligatory for growth on sulfide as hydrogen donor in photo and chemolithoautotrophic bacteria. It is a unique enzyme which is responsible for transfer of electrons from sulfide into the quinone pool. This enzyme converts the sulphide to Sulfur by the reaction.
Notes
During designing we used RFC10 assembly to create this biobrick by the use of previously existing biobricks. As per the protocol of RFC 10 we were constrained to use pSB1K3 as our backbone to eliminate the chances of false clone as our Part A and Part B were present in pSB1C3. Once cloning was done we digested our clone with EcoR1 and Pst1, and ligated it with pSB1C3 to standardise our part.Source
Biobrick Bba_K896000 and Constitutive Promoter (BBA_J23119)| Access | Instance | Definition |
| public public | BBa_J23119 BBa_K896000 | BBa_J23119 BBa_K896000 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23119 BBa_K896000 | 1,35 42,1322 | BBa_J23119 BBa_K896000 |