XenB

BBa_K1398000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1398000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Exeter iGEM 2014

Date created: 2014-08-06 11:00:00

Date modified: 2015-05-08 01:10:15

XenB (Xenobiotic Reductase B)

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1398000_sequence (Version 1)

Description

Xenobiotic Reductase B, created for use as a Trinitrotoluene and Nitroglycerine degrading protein.

A monomeric flavin that can reduce certain nitro- groups to nitrate-. Increases the resistance of organisms to the toxic effects of nitrocompounds.

This sequence encodes for a protein (with an attached His (x6) Tag to allow for purification) and nothing else. It therefore requires regulatory sequences (promoter/RBS/terminator) to be added for expression.

Notes

The original sequence of this part was found using the NCBI database and taken from Pseudomonas putida BIRD-1. The sequence was then codon-optimised for increased expression in E. coli. This has resulted in a novel synthetic sequence.

Source

Source of original sequence: Pseudomonas putida BIRD-1.

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1398000/1

Member of these Collections

• //chassis/prokaryote/ecoli
• //function/degradation
• iGEM Parts Registry