AgaA

BBa_K1403002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1403002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Cristina Garcia-Timermans

Date created: 2014-10-05 11:00:00

Date modified: 2015-05-08 01:10:17

AgaA expression (3M2H-> 3H3MH)

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1403002_sequence (Version 1)

Description

agaA is an enzyme present in Corynebacterium striatum that is the main responsible for body odor. It metabolizes 3-methyl-2-hexenoic acid into 3-hydroxy-3-methylhexanoic acid, that is a sulphure volatile compound.

Name: BBa_K1403002
Description: Biobrick submission of agaA expression construct
Resistances : Cm
Backbone : pSB1C3
Parts: Pcons - RBS - agaA - his tag
Design date: 11/June/2014

This part contains:

1) Plasmid pSB1C3: High copy BioBrick assembly plasmid. Constitutive expression. To use in E coli

2) agaA construct:
- Promoter BBa_J23108 from the Anderson's promoter collection

- RBS from the RBS Calculator of Salis lab specific for E coli

- BioBrick Prefix + Scar from the iGEM Parts webpage

- agaA sequence codon optimized for E coli 12 (IDT online tool). We checked with Geneious that there were no restriction sites for EcoRI, SpeI, XbaI and PstI.

- Histidine tag: 6 Histidines in C-terminal were added
-Stop codon
-BioBrick scar + BioBrick suffix from the iGEM Parts webpage

Notes

Codon optimization. Check no restriction sites for EcoRI, SpeI, XbaI and PstI.

Source

agaA sequence codon optimized for E coli 12 (IDT online tool)

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1403002/1

Member of these Collections

• iGEM Parts Registry