Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ye Chen
Date created: 2014-10-09 11:00:00
Date modified: 2015-05-08 01:10:18
LasR+LasR/PAI1 Inducible promoter+GFP+RhlR+RhlR/PAI2 Inducible promoter+RFP
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1407015_sequence (Version 1) |
Description
It's composed of seven sections:constitutive production of LasR,LasR/PAI1 Inducible promoter with RBS(B0034)and GFP,terminator,constitutive promoter,RhlR protein generator ,RhlR/PAI2 inducible promoter with RBS (B0030) and RFP,Terminator.When PAI1 exsits,the part could be inducibled and expresses GFP protein;When PAI2 exsits,the part could be inducibled and expresses RFP protein.Notes
None.Source
Existing parts on the registry.| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23100 BBa_K553003 BBa_B0034 conserved BBa_C0179 BBa_B0015 BBa_B0010 stem_loop BBa_B0012 T7 TE polya stop BBa_R0079 BBa_K575009 OP2 OP1 -35 -10 BBa_B0034 conserved GFP protein BBa_E0040 BBa_B0010 BBa_B0015 stem_loop BBa_B0012 T7 TE polya stop BBa_K1407008 BBa_J23119 BBa_I0466 BBa_B0034 conserved rhlR BBa_C0071 LVA Help:Barcodes BBa_B0010 stem_loop BBa_B0012 T7 TE polya stop RhlR BBa_R0071 BBa_K575014 -35 -10 start BBa_B0030 RBS-1\Strong RBS mrfp1 BBa_E1010 Help:Barcodes BBa_B0010 BBa_B0015 stem_loop BBa_B0012 T7 TE polya stop BBa_K1407011 | 1,35 1,921 44,55 48,51 62,784 793,921 793,872 804,847 881,921 888,907 908,921 914,914 930,1086 930,1832 975,992 1035,1054 1046,1051 1069,1074 1095,1106 1099,1102 1113,1832 1113,1832 1841,1920 1841,1969 1852,1895 1929,1969 1936,1955 1956,1969 1962,1962 1,1969 1978,2012 2021,2962 2021,2032 2025,2028 2039,2761 2039,2800 2762,2794 2801,2825 2834,2913 2845,2888 2922,2962 2929,2948 2949,2962 2955,2955 2971,2990 2971,3023 2971,3758 2989,2994 3012,3017 3023,3023 3032,3046 3032,3046 3039,3043 3053,3727 3053,3758 3734,3758 3767,3846 3767,3895 3778,3821 3855,3895 3862,3881 3882,3895 3888,3888 1978,3895 | engineered_region feature/BioBrick engineered_region feature/BioBrick engineered_region feature/BioBrick feature/conserved conserved_region engineered_region feature/BioBrick engineered_region feature/BioBrick feature/BioBrick engineered_region feature/stem_loop stem_loop feature/BioBrick engineered_region stem_loop feature/stem_loop polyA_site feature/polya feature/stop stop_codon feature/BioBrick engineered_region feature/BioBrick engineered_region feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site promoter feature/promoter promoter feature/promoter feature/BioBrick engineered_region feature/conserved conserved_region feature/protein CDS feature/BioBrick engineered_region feature/BioBrick engineered_region engineered_region feature/BioBrick stem_loop feature/stem_loop engineered_region feature/BioBrick feature/stem_loop stem_loop polyA_site feature/polya feature/stop stop_codon feature/BioBrick engineered_region engineered_region feature/BioBrick engineered_region feature/BioBrick feature/BioBrick engineered_region feature/conserved conserved_region feature/cds CDS engineered_region feature/BioBrick tag feature/tag feature/barcode engineered_tag engineered_region feature/BioBrick stem_loop feature/stem_loop feature/BioBrick engineered_region feature/stem_loop stem_loop polyA_site feature/polya stop_codon feature/stop feature/binding non_covalent_binding_site feature/BioBrick engineered_region engineered_region feature/BioBrick feature/promoter promoter feature/promoter promoter start_codon feature/start engineered_region feature/BioBrick ribosome_entry_site feature/rbs feature/rbs ribosome_entry_site CDS feature/cds feature/BioBrick engineered_region feature/barcode engineered_tag engineered_region feature/BioBrick feature/BioBrick engineered_region stem_loop feature/stem_loop engineered_region feature/BioBrick stem_loop feature/stem_loop polyA_site feature/polya stop_codon feature/stop engineered_region feature/BioBrick |