| Types | DnaRegion
|
| Roles | CDS
Coding
|
| Sequences | BBa_K1408001_sequence (Version 1)
|
Description
Gal4 protein binds to a Gal1 promoter while VP16 recruits transcription machinery promoting transcription to anything under the Gal1 promoter. The two must be co-localized to work as a transcriptional activator.
Notes
Xbe1, EcoR1 and Spe1 restriction sites were present in the original sequence obtained from the Fields Lab. The submitted part was synthesized with synonymous substitutions (silent mutations) amplified with primers containing the biobrick prefix and suffix and then digested and ligated into the iGEM vector.
Source
Gal4 is a DNA binding fragment from Saccharomyces cerevisiae. VP16 is a transcriptional activator from herpes simplex virus. This part was obtained from the Fields Lab at the University of Washington,
