BBa_K1421002

BBa_K1421002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1421002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Mengni Wang

Date created: 2014-10-03 11:00:00

Date modified: 2015-05-08 01:10:22

plux R+RBS+cI+2TE

Details

• Types: DnaRegion
• Roles: Device; engineered_region
• Sequences: BBa_K1421002_sequence (Version 1)

Description

This device is comprised of plux R (BBa_R0062), RBS (BBa_B0034), cI (Part:BBa_C0051) and 2 TE (BBa_B0010 and BBa_B0012).
The lux pR is a right promoter in the lux cassette of V. Fischeri, which gives weak constitutive expression while enhanced when binding to the complex of LuxR and AHL (acyl-homoserine lactone ).
CI is from BBa_C0051 which is designed by Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross. The cI repressor from E. coli phage lambda is modified with an LVA tail for rapid degradation of the protein. It binds to the cI regulator.

Notes

TO USE A SIMPLER WAY

Source

FROM PART POOL

Components

Access	Instance	Definition
public	BBa_R0062	lux pR
public	BBa_B0034	BBa_B0034
public	BBa_C0051	cI lam
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0062	1,55	lux pR
BBa_B0034	64,75	BBa_B0034
BBa_C0051	82,856	cI lam
BBa_B0010	865,944	BBa_B0010
BBa_B0012	953,993	BBa_B0012

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1421002/1

Member of these Collections

• iGEM Parts Registry