Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Casey Martin
Date created: 2014-10-16 11:00:00
Date modified: 2015-05-08 01:10:23
Lactose-induced Killswitch using antiholin Hyb5
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1422504_sequence (Version 1) |
Description
This is a killswitch designed for Bacillus subtilis. It is lactose and IPTG inducible, has a RBS that has high efficiency with Bacillus subtilis, the antiholin Hyb5 as a lysing agent, and a forward terminator.Hyb5 was chosen because it is effective on Bacillus subtilis at neutral pH and 37 Celsius. It is also effective on certain strains of E. coli and other bacteria.
Source: Wang, S.; Kong, J.; & Zhang, X. (19 November, 2008). Identification and characterization of the two-component cell lysis cassette encoded by temperate bacteriophage ϕPYB5 of Lactobacillus fermentum. Journal of Applied Microbiology, Volume 105, Issue 6, pages 1939-1944. DOI: 10.1111/j.1365-2672.2008.03953.x http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.2008.03953.x/full
Notes
This killswitch is intended for Bacillus subtilis, and as such uses a RBS that works well in Bacillus. The choice of lactose as a promoter was chosen because it penetrates soil, is economically feasible to spread across a field, is not a food source for Bacillus, and does not harm plants or the natural soil microbiome. Hyb5 was chosen as a lysing agent because it is effective on Bacillus subtilis at neutral pH around field temperature.Source
Hyb5 comes from NCBINCBI. (2 December, 2008). Lactobacillus phage YB5 holin gene, complete cds. http://www.ncbi.nlm.nih.gov/nuccore/EF623894?report=GenBank