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BBa_K1430000
BBa_K1430000
Version 1
Component
Source:
http://parts.igem.org/Part:BBa_K1430000
Generated By:
https://synbiohub.org/public/igem/igem2sbol/1
Created by: Cedric Bl??tz
Date created: 2014-09-29 11:00:00
Date modified: 2015-05-08 01:10:23
yeast 2-micron
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Details
Types
DnaRegion
Roles
Regulatory
promoter
Sequences
BBa_K1430000_sequence (Version 1)
Description
Mutated 2-micron for plasmid amplification in yeast cells. The 2 micron sequence was mutated that internal restriction sites (for Pre/-Suffix enzymes) are lost.
Notes
A silent mutation was created via PCR to delete restriction site for Xba1.
Source
pDEST-GAD (plasmid, Invitrogen)
Sequence Annotations
Sequence Annotation
Location
Component
/ Role(s)
2-micron ori
1,1343
sequence_feature
feature/misc
Other Properties
igem#sampleStatus
It's complicated
igem#status
Planning
synbiohub#ownedBy
user/james
synbiohub#ownedBy
user/myers
synbiohub#topLevel
BBa_K1430000/1
Member of these Collections
iGEM Parts Registry
Attachments
iGEM Main Page
b71660c3-4e3f-4753-b8d7-4014c89609bd
iGEM Design Page
c0315102-31cb-4778-8144-f920b29346ee
iGEM Experience Page
6c878a1b-5066-4592-a4bf-50908eb65b83
Component Sankey
3a7957f8-d360-4359-9641-e59bd5e2133b
Accurat VisBol
e00bd670-5749-4846-9131-d430c549e42b
Accurat SeqViz
5267bf9d-3993-448b-a724-c1e870855baa
Accurat Component Use
3a3da1fd-e51f-47ea-b7c4-4e4bd2d3b48b