BBa_K143069

BBa_K143069 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K143069
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chris Hirst
Date created: 2008-10-25 11:00:00
Date modified: 2015-05-08 01:10:24

AmyE integratable PoPS generator (Pveg-gsiB)



Types
DnaRegion

Roles
Generator

engineered_region

Sequences BBa_K143069_sequence (Version 1)

Description

AmyE 5' Integration sequence(BBa_K143001) coupled to the PoPS generator Pveg-gsiB (BBa_K143052).

The amyE 5' integration sequence allows integration into the B. subtilis genome at the amyE locus if the 3' amyE integration sequence(BBa_K143002) is cloned onto the 3' end of the construct.

The Pveg-gsiB promoter and RBS for B. subtilis constitutively generate a PoPS output.

Notes

The amyE 5' integration sequence was PCR cloned from the B. subtilis integration vector pDR111 utilising Pfu DNA polymerase. The sequence of Pveg-gsiB was obtained from papers.

Source

The amyE 5' integration sequence was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase and cloned into a BioBrick with the Pveg-gsiB that was synthesised by GeneArt

igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K143069/1