BBa_K143081

BBa_K143081 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K143081
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chris Hirst
Date created: 2008-10-27 12:00:00
Date modified: 2015-05-08 01:10:24

P43-spoVG RFP expression construct



Types
DnaRegion

Roles
Generator

engineered_region

Sequences BBa_K143081_sequence (Version 1)

Description

RFP gene under expresson of the P43 promoter and spoVG RBS of B. subtilis, with a chloramphenicol resistance marker for ease of selection.

Also contains the 5' and 3' amyE integration sequences to allow integration into B. subtilis at the amyE locus.

This device was used by the Imperial iGEM 2008 team to characterise the P43 promoter and spoVG RBS (Combined part BBa_K143051) as part of the Biofabricator subtilis project.

Notes

The 5' and 3' amyE integration sequences were PCR cloned from the B. subtilis integration vector pDR111 using Pfu DNA polymerase. The double terminator, RFP gene and chlorapmphenicol acetyltransferase gene were taken from the registry. The sequence of promoter P43 and RBS spoVG were taken from papers.

Source

The 5' and 3' amyE integration sequences were PCR cloned from the B. subtilis integration vector pDR111 utilising Pfu DNA polymerase and cloned into a BioBrick with the parts from the registry and the P43-spoVG part that was synthesised by GeneArt.

igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K143081/1