[OriTRP4]+

BBa_K1439001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1439001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Wenqi Li

Date created: 2014-09-30 11:00:00

Date modified: 2015-05-08 01:10:25

This part contains a reporter gene BBa_J04450, combined with OriTRP4. Used to test plasmid mobility.

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K1439001_sequence (Version 1)

Description

Once the conjugation take place,the recipient bacterium will obtain the reporter gene which gives us a signal to distinguish whether the conjugation has happened as well as the conjugation efficiency easily through observation. In addition, it facilitates the Follow-up experiments on the conjugation rate, conjugation time, and conjugation efficiency between different strains.

Notes

OriTRP4 region contains some promoters, we put it in the back of reporter gene . In order to reduce the impact on protein expression

Source

OriTRP4 comes form the RP4 plasmid, from 51098bp to 51448bp, reporter gene comes from BBa_J04450.

Components

Access	Instance	Definition
public	BBa_K1439000	OriTRP4
public	BBa_J04450	BBa_J04450

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1439000	1,350	OriTRP4
BBa_J04450	359,1427	BBa_J04450

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1439001/1

Member of these Collections

• iGEM Parts Registry