BBa_K1441002

BBa_K1441002 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1441002
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Julia Dave
Date created: 2014-10-02 11:00:00
Date modified: 2015-05-08 01:10:26

PGAPz alpha A + Linker



Types
DnaRegion

Roles
Plasmid_Backbone

plasmid_vector

Sequences BBa_K1441002_sequence (Version 1)

Description

This plasmid is PGAPzα C + modified Multiple Cloning Site(MCS) (Linker) fixed according to iGEM standards. We replaced old multiple cloning cite with new modified multiple cloning site, which has new cutting sites according to iGEM standard. The original MCS has ECORI, KpnI, NotI and XbaI restriction enzyme binding sites compared to the new MCS we designed in which there are ECORI, NotI, XbaI, SpeI and PstI restriction enzyme cutting sites. PGAPzα C + Linker has α-secretion factor before MCS which allows yeast cells and some Eukaryotic cells to secrete the protein into the outside of the cell membrane. Also after MCS there are MYC epitope and 6x histag that are used to tag the protein so it can be easily identified by westernblot technique.

Notes

We replaced old multiple cloning cite with new modified multiple cloning site, which has new cutting sites according to iGEM standard.

Source

None

igem#experience
Works
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/myers
 
synbiohub#ownedBy
user/james
 
synbiohub#topLevel
BBa_K1441002/1