BBa_K1444024

BBa_K1444024 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1444024
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dan Ziemianowicz
Date created: 2014-10-16 11:00:00
Date modified: 2015-05-08 01:10:27

Composite promoter and weak B.subtilis RBS - C1-434 x3



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_K1444024_sequence (Version 1)

Description

A composite part consisting of the constitutive promoter Pveg (BBa_K823003), followed by three copies of the repressible promoter C1-434 (BBa_R0052), and ending with the weak RBS from B. subtilis (BBa_K780002).

This composite part was intended to control the expression of a downstream reporter, regulated by the expression of the pLacI repressor protein. The constitutive promoter Pveg allows reliable expression when the repressible promoter is not repressed. The triplicate repressible promoter allows for stronger repression.

Notes

No interference with endogenous cell signalling.

Source

B. subtilis

igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1444024/1