BBa_K1444026

BBa_K1444026 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1444026
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dan Ziemianowicz
Date created: 2014-10-16 11:00:00
Date modified: 2015-05-08 01:10:27

Composite promoter and weak B.subtilis RBS - C2-lambda x3



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_K1444026_sequence (Version 1)

Description

A composite part consisting of the constitutive promoter Pveg BBa_K823003, followed by three copies of the repressible promoter C2-P22 BBa_R0051, and ending with the weak RBS from B. subtilis BBa_K780002.

This composite part was intended to control the expression of a downstream reporter, regulated by the expression of the C1-lambda repressor protein. The constitutive promoter Pveg allows reliable expression when the repressible promoter is not repressed. The triplicate repressible promoter allows for stronger repression.

Notes

No interference with endogenous cell signalling.

Source

B. subtilis

igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1444026/1