T7

BBa_K145014 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K145014

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Benjamien Moeyaert

Date created: 2008-07-23 11:00:00

Date modified: 2015-05-08 01:10:28

T7 RNA Polymerase with UmuD derived tag

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K145014_sequence (Version 1)

Description

T7 RNA Polymerase with N-terminal UmuD degradation tag.

Notes

This part is different from the simple composition of the tag and the polymerase, as there cannot be a stop codon in the scar.

Source

Part composed of the T7 RNA Polymerase and the N-terminal degradation tag, but with no scar (no stop codon)

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
start	1,3	feature/start, start_codon
stop	2740,2742	feature/stop, stop_codon
stop	2737,2739	stop_codon, feature/stop
tag	1,87	tag, feature/tag
cds	88,2736	feature/cds, CDS

Other Properties

• igem#experience: None
• igem#partStatus: Released HQ 2013
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K145014/1

Member of these Collections

• //cds/enzyme/rnapolymerase
• //chassis/bacteriophage/t7
• iGEM 2018 Cell E. coli strain NEB 10-beta
• iGEM 2018 Distribution Plate 4
• iGEM 2018 Distribution Plate 4 Well 1E
• iGEM 2018 Part Library
• iGEM 2018 Plasmid pSB1C3
• iGEM Parts Registry