BBa_K1453300

BBa_K1453300 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_K1453300

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Binhan Hao

Date created: 2014-10-15 11:00:00

Date modified: 2015-05-08 01:10:30

FL3-TALE

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1453300_sequence (Version 1)

Description

Since we cannot connect TALE by Golden Gate method designed by 2012 Freiburg, so the sequence was synthesized by Genwize company. This TALE can recognize the DNA sequence TTGGTCATGAGA(12bp). Moreover, we use this part with our part BBa_K14530000 to make our composite part BBa_K1453305.

Notes

There are a flexible linker 3 before the TALE protein.

Source

This part is synthesized by company.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
start codon	1,3	start_codon, feature/start
flexible linker 3	4,60	CDS, feature/cds
TALE	61,1488	CDS, feature/cds
His-tag	1489,1506	feature/tag, tag
stop codon	1507,1509	stop_codon, feature/stop

Other Properties

• igem#experience: None
• igem#sampleStatus: Discontinued
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1453300/1

Member of these Collections

• iGEM Parts Registry