Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Mustafa Yılmaz
Date created: 2014-10-22 11:00:00
Date modified: 2015-05-08 01:10:32
tetR-ODD-VP16
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1456025_sequence (Version 1) |
Description
ODD (oxygen dependent degradation) domain, which is present in the HIF-1α (hypoxia inducible factor) protein that it activates in lower oxygen levels and breaks down in intermediate oxygen levels, is a protein domain that plays a key regulatory role in the transcription of the HIF-1α factor. In intermediate oxygen levels, the ODD domain of the HIF-1α protein is hydroxilized by the hydroxilase enzyme and the hydroxilized HIF-1α enzyme breaks down through ubiquitin attachment. Thus, the ODD causes the regulation of a transcription factor, which is active in hypoxic conditions and inactive in normoxic conditions. The Tet-Off is a strong system composed of two strong plasmids. Of the two plasmids that form this system, the TetR-VP16 fusion protein produced by the first plasmid acts as a transcriptionary factor regulating the Tet operator sequence of the second plasmid(pTRE). (TetR: DNA Binding Domain, DBD; VP16: Transactivating domain, TAD).The Tet-Off system can be inhibited using tetracyclane.
In this part, through placing the ODD region of the HIF-1α in between the synthetic TetR - VP16 transcription factors (which are not present in mammallian cells and have been used in molecular biology experiments for a long time), the transcription factor was designed to gain sensitivity to oxygen.
Notes
noneSource
human| Access | Instance | Definition |
| public public public | BBa_K1456026 BBa_K1456005 BBa_K1150001 | BBa_K1456026 ODD BBa_K1150001 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1456026 BBa_K1456005 BBa_K1150001 | 1,765 766,1527 1528,1899 | BBa_K1456026 ODD BBa_K1150001 |