BBa_K1459002

BBa_K1459002 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1459002
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Grzegorz Ścibisz
Date created: 2014-09-30 11:00:00
Date modified: 2015-05-08 01:10:32

C-term of PmrB from Salmonella enterica



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K1459002_sequence (Version 1)

Description

PmrB is a transmembrane kinase. After binding iron (III) ion by binding peptide on extracellular loop, it's intracellular domain gains kinase activity and phosphorylates PmrA (BBa_K1459000).

PmrB C-term is a part of two-component system. When fused with some binding tag, PmrB(N-term), PmrA and pmrC-reporter, it is a viable detecting system.

Notes

We had to use Silver (RFC 23) Standard instead of RFC 10 to allow protein fusions.

Source

We obtained PmrA-PmrB thanks to kindness of prof. He from the University of Chicago.

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BBa_K1459002/1