BBa_K1459006

BBa_K1459006 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1459006

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Grzegorz Ścibisz

Date created: 2014-10-06 11:00:00

Date modified: 2015-05-08 01:10:32

pmrC

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K1459006_sequence (Version 1)

Description

This is pmrC promoter native to Salmonella enterica.
PmrA-PmrB two-component system is native to Salmonella enterica and in it's native state it is responsible for chemotaxis. PmrB is a transmembrane protein with iron binding peptide on it's extracellular loop. When PmrB binds iron (III) iron, it's intracellular domain gains kinase activity and phosphorylates PmrA, which subsequently binds to pmrC promoter and induces expression of chemotaxis CheZ protein. In this part iron binding tag on the extracellular loop was exchanged with a lanthanide binding tag (LBT), to allow PmrA-PmrB two-component system to respond to lanthanide ions.

Notes

This is pmrC sequence derived from bioinformatic sources, after search for promotor at 5' end of CheZ protein.

Source

We obtained the pmrC-GFP sequence thanks to kindness of prof. He from University of Chicago.

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1459006/1

Member of these Collections

• iGEM Parts Registry