Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Grzegorz Ścibisz
Date created: 2014-10-06 11:00:00
Date modified: 2015-05-08 01:10:32
PmrB(LBT) from Salmonella
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1459010_sequence (Version 1) |
Description
PmrB(LBT) is a engineered PmrB gene, in which iron binding tag was replaced by lanthanide binding tag.PmrA-PmrB two-component system is native to Salmonella enterica and in it's native state it is responsible for chemotaxis. PmrB is a transmembrane protein with iron binding peptide on it's extracellular loop. When PmrB binds iron (III) iron, it's intracellular domain gains kinase activity and phosphorylates PmrA, which subsequently binds to pmrC promoter and induces expression of chemotaxis CheZ protein. In this part iron binding tag on the extracellular loop was exchanged with a lanthanide binding tag (LBT), to allow PmrA-PmrB two-component system to respond to lanthanide ions.