Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nikolas Layer, Philipp H. O. Mayer and Philip Roessler
Date created: 2014-10-05 11:00:00
Date modified: 2015-05-08 01:10:41
Ssp GyrB Split Intein
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1483003_sequence (Version 1) |
Description
N-Intein part of the ssp GyrB split intein.Can be used in conjunction with the 6 amino acid long C-Intein to N-terminally immobilize a peptide onto a matrix.
For immobilisation, parts need to be fused to the C-terminus of the N-intein. The N-terminus of the C-Intein is fused the matrix. The split intein recombines and splices itself out, thereby specificially immobilising the N-terminus of the peptide to the matrix.
The sequence of the corresponding C-Intein is: H2N-GVPVHN-COOH
(was aquired by peptide synthesis and is thus not part of the registry)
This part can also be used in order to create fusion proteins, by attaching the C-Intein to a proein instead of the matrix.
Part in RFC25 standard.