BBa_K1487010

BBa_K1487010 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1487010
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Samantha Bermudez
Date created: 2014-10-01 11:00:00
Date modified: 2015-05-08 01:10:43

Guide RNA (gRNA) target for tcpE



    • Details

    Types
    DnaRegion

    Roles
    mature_transcript_region

    RNA

    Sequences BBa_K1487010_sequence (Version 1)

    Description

    This part contains guide RNA (gRNA) that can target toxin coregulated pilus E (tcpE) gene.

    Notes

    Target sequence must be 20 base pairs and be flanked with a PAM sequence (NGG) on the 3' end in order for Cas9 + gRNA to successfully target and cleave the sequence.

    Source

    Part was assembled using complementary primers to match a 20 base pair sequence in the tcpE coding region.

    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1487010/1
     

    Attachments

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