BBa_K1487030

BBa_K1487030 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1487030
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Samantha Bermudez
Date created: 2014-10-02 11:00:00
Date modified: 2015-05-08 01:10:43

Promoter + Guide RNA (gRNA) target for tcpD



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K1487030_sequence (Version 1)

Description

This part contains guide RNA (gRNA) that can target toxin coregulated pilus D (tcpD) gene under strong constitutive promoter J23119.

Notes

Target sequence must be 20 base pairs and be flanked with a PAM sequence (NGG) on the 3' end in order for Cas9 + gRNA to successfully target and cleave the sequence.

Source

Part was assembled using complementary primers to match a 20 base pair sequence in the tcpD coding region.

igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1487030/1