BBa_K1487041

BBa_K1487041 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1487041

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Samantha Bermudez

Date created: 2014-10-02 11:00:00

Date modified: 2015-05-08 01:10:43

Promoter + Guide RNA (gRNA) target for toxT

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K1487041_sequence (Version 1)

Description

This part contains guide RNA (gRNA) that can target transcriptional regulator toxT (toxT) gene under strong constitutive promoter J23119.

Notes

Target sequence must be 20 base pairs and be flanked with a PAM sequence (NGG) on the 3' end in order for Cas9 + gRNA to successfully target and cleave the sequence.

Source

Part was assembled using complementary primers to match a 20 base pair sequence in the toxT coding region.

Components

Access	Instance	Definition
public	BBa_J23119	BBa_J23119
public	BBa_K1487020	BBa_K1487020

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J23119	1,35	BBa_J23119
BBa_K1487020	44,145	BBa_K1487020

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1487041/1

Member of these Collections

• iGEM Parts Registry