BBa_K1497017

BBa_K1497017 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1497017

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen

Date created: 2014-10-06 11:00:00

Date modified: 2015-05-08 01:10:45

Naringenin producing operon under the T7 promoter BBa_I712074

Details

• Types: DnaRegion
• Roles: engineered_region; Generator
• Sequences: BBa_K1497017_sequence (Version 1)

Description

Transformed into BL21(DE3) and induced with IPTG this composite is able to produce naringenin from tyrosine.
It consists of 4 genes:

4-CL (BBa_K801093)
TAL (BBa_K1033000)
CHI (BBa_K1497000)
CHS (BBa_K1497001)

For a strong and stable translation in front of each gene the RBS Bba_B0034 is implemented.

For characterization the naringenin biosensor K1497020 was used. After 24 h incubation GFP fluorescence was visible to the unaided eye. We measured the relative GFP fluorescence by use of OptimaFluoroStar.

Notes

d

Source

d

Components

Access	Instance	Definition
public	BBa_I712074	BBa_I712074
public	BBa_K1497007	BBa_K1497007

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I712074	1,46	BBa_I712074
BBa_K1497007	55,5357	BBa_K1497007

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1497017/1

Member of these Collections

• //cds/biosynthesis
• //cds/biosynthesis/anthocyanins
• iGEM Parts Registry