BBa_K1498000

BBa_K1498000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1498000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Charlotte Green
Date created: 2014-10-06 11:00:00
Date modified: 2015-05-08 01:10:46

kerUS keratinase from Brevibacillus brevis



    • Details

    Types
    DnaRegion

    Roles
    CDS

    Coding

    Sequences BBa_K1498000_sequence (Version 1)

    Description

    Coding region of kerUS keratinase from Brevibacillus brevis with signal for extracellular secretion, which is cleaved following secretion

    Codes for a 45.9kDa (predicted)pre-processed protein, cleaved to 29kDa active protein

    Notes

    codon optimisation for E. coli, ensuring no BioBrick restriction enzyme sites were present

    Source

    is the codon optimised (for E. coli) kerUS gene from:
    Jaouadi, N. Z., Rekik, H., Badis, A., Trabelsi, S., Belhoul, M., Yahiaoui, A. B., ??? Jaouadi, B. (2013). Biochemical and molecular characterization of a serine keratinase from Brevibacillus brevis US575 with promising keratin-biodegradation and hide-dehairing activities. PloS one, 8(10), e76722. doi:10.1371/journal.pone.0076722

    Genbank Number: KC152966

    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1498000/1
     

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