BBa_K1499253

BBa_K1499253 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1499253
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Raman Nelakanti
Date created: 2014-10-07 11:00:00
Date modified: 2015-05-08 01:10:46

aeBlue generator with 3 UAG stops + supP tRNA



    • Details

    Types
    DnaRegion

    Roles
    engineered_region

    Generator

    Sequences BBa_K1499253_sequence (Version 1)

    Description

    todo

    Notes

    added 3 stop codons for the amberless system

    Source

    iGEM

    Sequence Annotation Location Component / Role(s)
    J23104
    B0034
    RFC10 scar
    spacer
    FLAG-tag
    aeBlue (K864401, re-codon optimized)
    TAG stop
    TAG stop 2
    TAG stop 3
    His-tag
    double stop codon
    Terminator (B0010)
    supP tRNA (K1499251)
    		1,35
    44,55
    36,43
    56,61
    62,88
    92,775
    260,262
    416,418
    596,598
    776,793
    794,799
    800,879
    880,1103
    		feature/promoter promoter
    ribosome_entry_site feature/rbs
    feature/scar
    feature/misc sequence_feature
    feature/tag tag
    feature/cds CDS
    stop_codon feature/stop
    feature/stop stop_codon
    feature/stop stop_codon
    feature/tag tag
    feature/stop stop_codon
    feature/stem_loop stem_loop
    feature/BioBrick engineered_region

    igem#experience
    Works
     
    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1499253/1
     

    Attachments

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