BBa_K1500014

BBa_K1500014 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1500014
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ryan Beckner
Date created: 2014-10-16 11:00:00
Date modified: 2015-05-08 01:10:46

paroF->MCherry-MutH(E56A)



Types
DnaRegion

Roles
Translational_Unit

engineered_region

Sequences BBa_K1500014_sequence (Version 1)

Description

This biobrick contains mutH() and mCherry under the control of the tyrosine-sensitive promoter paroF. Unless the E. coli cell produces very high levels of tyrosine, the mutagenizing protein mutH() and mCherry will be produced. The mCherry is used as a reporter to indicate when this maximum tyrosine production is reached. mutH should cause genomic mutations that should result in the cell eventually producing high levels of tyrosine.

Notes

NA

Source

paroF, mutH are genomic XL1Blue, mCherry is from distribution kit.

igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1500014/1