Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Matthew Ykema
Date created: 2014-10-16 11:00:00
Date modified: 2015-05-08 01:10:47
accD portion of Acyl-CoA Ligase
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1502003_sequence (Version 1) |
Description
This sequence codes for the accD protein. This must be combined with accA,accB, and accC in order to come together to produce the acyl-CoA ligase enzyme. This enzyme takes in free fatty acids and produces fatty acyl-CoA's.Notes
The PCR process included X and P overhangs in order to include into the pSB1C3 plasmid. A EcoRI site had to be removed from the sequence at the 745 bp mark.Source
This part was isolated through PCR from the pACM4-ACA plasmid ordered from AddGene.| Sequence Annotation | Location | Component / Role(s) |
| Start codon | 1,3 | start_codon feature/start |