Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sam Krug, Clay Swackhamer, Ashlee Smith, Emily Sileo
Date created: 2014-09-30 11:00:00
Date modified: 2015-05-08 01:10:47
Optimized Superfolder GFP - only fast codons
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1506002_sequence (Version 1) |
Description
Superfolder GFP has been modified so that the coding sequence contains all "fast" codons. The reason that we designed this version of superfolder GFP was to explore the possibilities with codon optimization. Currently, codon optimization focuses on whether the relative abundance of a codon in the genome. We have decided to extend this to whether codons for the specific amino acid are considered to have a fast translation rate or a slow translation rate; this variation of superfolder GFP has the fast coding sequence. We have modified the superfolder GFP so that there can be more expression of superfolder GFP.Notes
Our definition of fast codons was based on current research being done in our lab. There is data to support this definition and this is what we used to design the experiment.Source
We designed the coding sequence in MatLab using the software to break up the initial coding sequence and then substitute each codon for the fastest translation rate for that specific amino acid. After these were designed, we ordered them as gBLOCKS from IDT DNA.| Sequence Annotation | Location | Component / Role(s) |
| start superfolder GFP coding region stop BBa_I746916 | 1,3 1,720 715,720 1,720 | start_codon feature/start CDS feature/protein stop_codon feature/stop feature/BioBrick engineered_region |