BBa_K152003

BBa_K152003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K152003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: David Johnston Monje

Date created: 2008-10-30 12:00:00

Date modified: 2015-05-08 01:10:49

CrtY + RBS

Details

• Types: DnaRegion
• Roles: engineered_region; Translational_Unit
• Sequences: BBa_K152003_sequence (Version 1)

Description

crtE from Erwinia uredovora with a rbs preceeding it. This gene codes for lycopene cyclase which is the third step in the beta carotene synthesis pathway. In combination with CrtE and B, this works to produce lycopene, a red, antioxidant, carotenoid.

Notes

We needed to use site directed mutagenesis to add the SpeI site after the stop codon.

Source

The plasmid pUC-EUY which was sent to us by Ethan Johnson on iGEM Minnesota team timebomb.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
rbs	4,10	ribosome_entry_site, feature/rbs
cds	18,1166	feature/cds, CDS

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K152003/1

Member of these Collections

• //function/biosynthesis/isoprenoid
• iGEM Parts Registry