BBa_K1521000

BBa_K1521000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1521000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Danilo Keiji Zampronio

Date created: 2014-09-30 11:00:00

Date modified: 2015-05-08 01:10:49

Quorum threshold expression element (qteE) from Pseudomonas aeruginosa + SpoVg RBS

Details

• Types: DnaRegion
• Roles: engineered_region; Translational_Unit
• Sequences: BBa_K1521000_sequence (Version 1)

Description

The qteE is the main quorum sensing regulator in Pseudomonas Aeruginosa. In this bacteria it is expressed as a constitutive protein that interacts with other quorum sensing proteins, such as LasR, inhibiting them from their regulation role (inducer, inhibitor and other functions). Assemblying this protein with specific promoters we are able to control the quorum sensing threshold, which is and interesting feature for pathways activation control. Moreover, if we assemble the qteE with an inducible promoter like Plac, it is possible to induce it with different IPTG concentrations, creating distinct threshold for protein production screening and even quantitative purposes.

This conding sequence comes with the SpoVg RBS (BBa_K143021) for usage in Bacillus subtilis. We tested it in E. coli and it worked as well.

Notes

For appropriate use of this part as a threshold producer its important to test the molar ratio qteE:QSprotein in wich the inhibition caused by the QteE is on the verge of disappearing, so that you can set the threshold for your purpose correctly. If your putting qteE production under a inducible/repressible promoter it would be wise to do a concentration gradient for determination of the appropriate threshold.

Source

This sequence was amplified through PCR from the Pseudomonas Aeruginosa PA14 cDNA.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
SpoVg RBS	1,12	ribosome_entry_site, feature/rbs
qteE	19,591	feature/cds, CDS

Other Properties

• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1521000/1

Member of these Collections

• iGEM Parts Registry