UV Sensor

BBa_K154000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K154000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Mary Jane Stine

Date created: 2008-10-20 11:00:00

Date modified: 2015-05-08 01:10:52

recA promoter (J22106) + LacZ gene (I732017): DNA damage sensor

Details

• Types: DnaRegion
• Roles: engineered_region; Measurement
• Sequences: BBa_K154000_sequence (Version 1)

Description

This part combines the promoter of the recA gene (an SOS promoter) and the full LacZ gene, with Amp resistance. Original part numbers are J22106 (recA) and I732017 (lacZ). The promoter is in front of the LacZ gene, and therefore signals transcription of LacZ when the SOS DNA repair mechanism is triggered by massive DNA damage. LacZ encodes for beta-galactosidase, which cleaves lactose and its homolog X-gal. X-gal is cleaved into galactose and an insoluble blue dye. By plating on an X-gal plate, a bacterium with this plasmid should turn blue when exposed to extensive amounts of DNA damage.

Notes

N/A

Source

Source: Biobricks and the Registry of Standard Biological Parts
recA promoter (SOS promoter): part J22106
lacZ gene: part I732017

Components

Access	Instance	Definition
public	BBa_J22106	BBa_J22106
public	BBa_B0034	BBa_B0034
public	BBa_I732005	LacZ

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J22106	1,192	BBa_J22106
BBa_B0034	201,212	BBa_B0034
BBa_I732005	219,3293	LacZ

Other Properties

• igem#experience: Issues
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K154000/1

Member of these Collections

• iGEM Parts Registry