Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Valencia_UPV team members
Date created: 2014-09-28 11:00:00
Date modified: 2015-06-09 09:45:45
Yellow biosafety module for plants
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K1554004_sequence (Version 1) |
Description
This biosafety device for plants consists on two submodules: a male sterility submodule and an identity preservation submodule. The male sterility submodule consist on a barnase specifically expressed in anthers under the regulation of the TA29 tapetum-specific promoter. As result, pollen from these plants is not fertile. The identity preservation submodule expresses the yellow chromoprotein AmilGFP, so that plants can be visually differentiated from non-transgenic plants.Notes
The assembly was performed by GoldenBraid assembly. The barnase was ampliified by PCR from Part:BBa_J72173 and a mutation in T105P was introduced.Source
TA29 promoter Part:BBa_K1554000Barnase Part:BBa_J72173
AmilGFP [Waiting for Norwich team part name in registry]
P35s Part:BBa_K1537015