BBa_K157003

BBa_K157003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K157003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: iGEM Team Freiburg 2008

Date created: 2008-10-25 11:00:00

Date modified: 2015-05-08 01:10:54

Anti-NIP singlechain Fv-Fragment

Details

• Types: DnaRegion
• Roles: Protein_Domain; polypeptide_domain
• Sequences: BBa_K157003_sequence (Version 1)

Description

Singlechain Fv.Fragment of an Anti-Nitro-Iodo-Phenol-antibody B1-8. Binds the hapten NIP (Nitro-Iodo-Phenole) with an affinity of 2.0 uM[1,2]. Designed for fusion to proteins or peptides via in frame cloning.

Notes

Between BioBrick 1.0 sites this part is flanked 5' by NgoMI(=NgoMIV) and 3' AgeI(=PinAI) to facilitate in frame cloning for protein fusions.

Source

Gene synthesis by GeneArt, optimized for expression in homo sapiens.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Anti-NIP sc Fv-fragment	1,735	feature/cds, CDS

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K157003/1

Member of these Collections

• //proteindomain/binding
• //proteindomain/internal
• iGEM Parts Registry