BBa_K157010

BBa_K157010 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K157010
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: iGEM Team Freiburg 2008
Date created: 2008-10-25 11:00:00
Date modified: 2015-05-08 01:10:54

glycine-serine linker fused to B-cell receptor transmembrane region
displays protein on cell surfac



Types
DnaRegion

Roles
polypeptide_domain

Protein_Domain

Sequences BBa_K157010_sequence (Version 1)

Description

Helical single-span transmembrane region of the B-Cell-Receptor with a flexible 15 aa Linker at the N-terminus. Designed for fusion to proteins or peptides that are to be presented at the cells surface; signal peptide for membrane integration (e.g. part Bba_K157001) required at the N-terminus of the whole construct!

Notes

Between BioBrick 1.0 sites this part is flanked 5' by NgoMI(=NgoMIV) and 3' AgeI(=PinAI) to facilitate in frame cloning for protein fusions.

Source

Gene synthesis by GeneArt, optimized for expression in homo sapiens.

Sequence Annotation Location Component / Role(s)
GGGGSx3-BCR-Transmembrane
1,108
feature/protein CDS
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K157010/1