SP-AntiNIP

BBa_K157029 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K157029
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: iGEM Team Freiburg 2008
Date created: 2008-10-25 11:00:00
Date modified: 2015-05-08 01:10:55

SP-AntiNIP-GGGSlinker-Tm-nCFP



Types
DnaRegion

Roles
polypeptide_domain

Protein_Domain

Sequences BBa_K157029_sequence (Version 1)

Description

Part consists of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker (extracellular part), the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split fluorophor YFP with a fluolinker joined to its N-Terminus (intracellular part).

Notes

none

Source

Derived by cloning of the parts BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002, BBa_K157006. For further information see the description of the parts mentioned above.

Sequence Annotation Location Component / Role(s)
NgoMI
AgeI
SP-AntiNIP-GGGSlinker-Tm-nCFP
SP
Scar (Thr, Gly)
Sc-Fv_anti-NIP
Scar (Thr, Gly)
GGGS-Linker
Scar (Thr, Gly)
Tm
Scar (Thr, Gly)
N-Term_CFP
4,9
1420,1425
1,1425
10,81
82,87
88,822
823,828
829,873
874,879
880,948
949,954
955,1419
feature/misc sequence_feature
feature/misc sequence_feature
feature/cds CDS
CDS feature/protein
feature/misc sequence_feature
feature/protein CDS
sequence_feature feature/misc
feature/protein CDS
sequence_feature feature/misc
feature/protein CDS
feature/misc sequence_feature
feature/protein CDS
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K157029/1