BBa_K1583013

BBa_K1583013 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1583013

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Stefan Robert Marsden

Date created: 2015-09-03 11:00:00

Date modified: 2015-09-18 06:57:49

CsgA missing the start codon for fusion protein

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1583013_sequence (Version 1)

Description

In order to create a fusion protein of CsgA with Mfp5 in front of it, CsgA was designed without its start codon.

Notes

An illegal PstI restriction site (766) in CsgA had to be mutated (A->G) not changing the protein sequence.

Source

This DNA was synthesized. The design was based on the paper "Strong underwater adhesives made by self-assembling multi-protein nanofibres".[1]
1. C.Zhong, T.Gurry, A.Cheng, J.Downey, Z.Deng, C. Stultz, T.Lu, Nature Nanotechnology, 2014, 9, 858-866.
An illegal PstI restriction site (766) in CsgA had to be mutated (A->G) not changing the protein sequence.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
CsgA	1,393	CDS, feature/cds

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1583013/1

Member of these Collections

• iGEM Parts Registry