BBa_K1583018

BBa_K1583018 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1583018

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Max van 't Hof

Date created: 2015-09-13 11:00:00

Date modified: 2015-09-14 01:44:54

SpyCatcher without startcodon + His-tag

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1583018_sequence (Version 1)

Description

SpyCatcher is a protein that can spontaneously form covalent isopeptide linkages with it??s counterpart `Spytag` under physiological conditions.

Notes

Startcodon was not included, since BFP (BBa_K1583017) was placed in front of this biobrick.

Source

This gene was synthesized as part of biobrick BBa_K1583113. The idea of using Spytag/SpyCatcher was based on the paper "Programmable biofilm-based materials fromengineered curli nanofibres"[1]

1. Nguyen, P. Q., Botyanszki, Z., Tay, P. K. R., & Joshi, N. S. (2014). Programmable biofilm-based materials from engineered curli nanofibres. Nature communications, 5.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
SpyCatcher	1,387	feature/protein, CDS
His-tag	388,405	non_covalent_binding_site, feature/binding
Stop-codon	406,408	stop_codon, feature/stop

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1583018/1

Member of these Collections

• iGEM Parts Registry