BBa_K1583106

BBa_K1583106 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1583106
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Stefan Robert Marsden
Date created: 2015-09-07 11:00:00
Date modified: 2016-01-25 11:30:44

pRha + CsgA & pTet + RFP



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K1583106_sequence (Version 1)

Description

Upon induction with rhamnose, CsgA is expressed. These protein monomers can aggregate to form amyloid nanofibres.
In order to better visualize the individual cells in the biofilm, we added the constitutive expression of RFP to our plasmid in a different operon. For our design, we took advantage of the double terminator present in the biobrick I13521 by placing it in front of our genes.

Notes

For our design, we took advantage of the double terminator present in the biobrick I13521 by placing it in front of our genes.

Source

The DNA was synthesized. The sequence originates from the genomic DNA of E. coli K-12 MG1655 (http://www.ncbi.nlm.nih.gov/gene/949055).

igem#experience
Works
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1583106/1