Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Seung Meen Choi, Brittany Lee, Nu Ri Choi, SOOJI LEE, Hyun Min Park
Date created: 2015-08-30 11:00:00
Date modified: 2015-09-17 07:52:24
Constitutive LuxR plus AHL-inducible LacZ
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_K1584035_sequence (Version 1) |
Description
It produces two proteins, first LuxR and then LacZ. LuxR is expressed constitutively. When AHL quorum sensing molecule is present, it activates LuxR. Then, the resulting AHL-LuxR compound would bind to the LuxPR promoter, producing LacZ. LacZ encodes the alpha fragment of beta galactosidase and, in the presence of X-gal, can produce a blue colored molecule in DH5alpha, DH10B and Top10 bacterial strains.Notes
There are few design considerations concerning BBa_K1584035. While cutting the EcoRI and Xbal sites there is a chance that the promoter might be cut off. Therefore, one would need to be extra careful when cutting the sites. Also, after the LuxR a terminator is always required. If there is no terminator, then the LacZ would not function.Source
J61100, C0062, C0060, K823017, R0062, I732006| Access | Instance | Definition |
| public public public public public | BBa_J61100 BBa_C0062 BBa_K823017 BBa_R0062 BBa_I732006 | BBa_J61100 luxr BBa_K823017 lux pR lacZ-alpha |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J61100 BBa_C0062 BBa_K823017 BBa_R0062 BBa_I732006 | 1,12 19,774 808,902 911,965 972,1205 | BBa_J61100 luxr BBa_K823017 lux pR lacZ-alpha |