Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Manuel Blank
Date created: 2015-09-07 11:00:00
Date modified: 2015-09-18 03:45:40
Human Haptoglobin
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1590002_sequence (Version 1) |
Description
Haptoglobin is a binding protein with high affinity for free Haemoglobin.Notes
Choosing the correct codon-bias, remove 'illegal' restriction sites, ensure that (stop)codons are in frame, addition of standard prefix and suffix.Primers for cloning Haptoglobin into overexpression vector pQE80-L.
Forward: 5' GCGC GGATCC TCTGCGCTGGGTGCGGTT 3'
Reverse: 5' GCGC GGTACC TTAGTTTTCCGCGATGGT 3'
Primers for cloning Haptoglobin into vector for bacterial 2-hybrid system, pUT18.
Forward: 5' GCGC GGATCC ATGTCTGCGCTGGGTGCG 3'
Reverse: 5' GCGC GAATTC GAGTTTTCCGCGATGGTTTTC 3'
Primers for cloning Haptoglobin into vector for bacterial 2-hybrid system, pT25.
Forward: 5' GCGC GGTACC TTAGTTTTCCGCGATGGT 3'
Reverse: 5' GCGC GGATCC ATGAACCTGCTGTCTCCGATCCTGTCT 3'
Part was synthesised with appropriate prefix/suffix sequences, hence no separate primers were required for cloning it into pSB1C3.
Source
In silico optimised version for E. coli K12 strains. Sequence ordered through IDT.| Sequence Annotation | Location | Component / Role(s) |
| ATG start codon Human Haptoglobin Protein Coding Sequence TAA stop codon | 1,3 1,1218 1219,1221 | start_codon feature/start CDS feature/cds stop_codon feature/stop |