Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Marieke Mulder
Date created: 2015-09-13 11:00:00
Date modified: 2015-09-14 05:40:32
tasA, amyloid-like fibers
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1597002_sequence (Version 1) |
Description
tasA is resposible for the amyloid-like fibers that are one of the main constituents of the extracellular matrix of Bacillus subtilis biofilms. After the expression of the tapA-sipW-tasA operon, TasA amyloid-like fibres are synthesised. These fibers attach to the cell wall. Together with extracellular polysaccharides, TasA amyloid-like fibers promote formation of cell clusters, where these amyloid-like fibers resemble bundles of cell chains. This effect results in a more robust biofilm.Notes
We had to change nucleotide 39 from a thymine into a adenine to get rid of a PstI restriction site and nucleotide 153 from a thymine into a cytosine to get rid of an EcoRI restriction site. The amino acid sequence wasn???t changed.Source
genomic sequence from Bacillus subtilis 168| Sequence Annotation | Location | Component / Role(s) |
| tasA original RBS from tasA | 21,809 1,20 | CDS feature/cds feature/rbs ribosome_entry_site |