BBa_K1598005

BBa_K1598005 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1598005
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Marta Napiorkowska
Date created: 2015-09-16 11:00:00
Date modified: 2015-09-17 09:55:11

pYear-RBS-TPH1-6xHis-Terminator



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K1598005_sequence (Version 1)

Description

Human tryptophan hydroxylase 1 gene under the control of Nitrate-sensitive pYear promoter

Notes

- human TPH1 gene was codon-optimized for E.coli and provided with C-terminal 6xHis tag
- sequence was synthesized in the form of gBlock and cloned into pSB1C3

Source

DNA synthesis

Sequence Annotation Location Component / Role(s)
pYear
RBS B0034
TPH1-6xHis
Terminator B0015
1,100
101,113
119,1474
1483,1611
feature/promoter promoter
ribosome_entry_site feature/rbs
CDS feature/cds
stop_codon feature/stop
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1598005/1